RNA interference (RNAi) is a naturally occurring process that involves the inhibition of gene expression at the post-transcriptional level. It is mediated by small RNA molecules, such as short interfering RNA (siRNA) and microRNA (miRNA), which bind to specific messenger RNA (mRNA) molecules and induce their degradation or translational repression. RNAi plays a critical role in many biological processes, including development, cell differentiation, and defense against viral infections.
The RNAi pathway can be harnessed for therapeutic purposes, such as gene therapy and drug discovery. However, the success of RNAi-based therapies depends on efficient and specific delivery of the small RNA molecules to the target cells or tissues.
RNAi targeted protein degradation has many potential applications in drug discovery and development. It can be used to selectively degrade disease-causing proteins, such as mutant or overexpressed proteins, which are often difficult to target using traditional small-molecule inhibitors.
ALL Chemistry Inc. provides RNA interference (RNAi) targeted protein degradation service that combines the RNAi pathway with the ubiquitin-proteasome system to induce targeted protein degradation. This approach involves the use of small RNA molecules, such as short interfering RNA (siRNA) or microRNA (miRNA), to selectively degrade specific target proteins in cells. The RNAi molecules are designed to specifically recognize and bind to the target mRNA, leading to its degradation, which in turn results in the degradation of the target protein.
Our services include:
Design and synthesis of siRNA or miRNA: The first step is to design and synthesize small RNA molecules that are complementary to the target mRNA. These small RNA molecules are typically 20-25 nucleotides in length and are designed to specifically recognize and bind to the target mRNA.
Delivery of siRNA or miRNA: The next step is to deliver the designed RNA molecules to the target cells or tissues. This can be done through various methods, such as electroporation, transfection, or viral vectors.
Formation of RNA-induced silencing complex (RISC): Once the RNA molecules are delivered to the cells, they are incorporated into the RNA-induced silencing complex (RISC), which binds to the target mRNA and induces its degradation.
Ubiquitination and degradation of the target protein: The degradation of the target mRNA leads to the degradation of the target protein by the ubiquitin-proteasome system.
Monitoring protein degradation: The effectiveness of RNAi-targeted protein degradation can be monitored by measuring the reduction in protein expression levels using various techniques, such as western blotting and immunofluorescence.
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